Inverter

Part:BBa_J5516

Designed by: Charles Yoon   Group: iGEM06_Toronto   (2006-10-29)

Arabinose -> LacI ts -| LacI+pL

WARNING: This is not a PoPS-based inverter! There are several other LacI inverters available from the Registry that send and receive signals via PoPS.

Increasing concentration of Arabinose should increase LacI ts output. As LacI ts forms a tetramer, it will repress the pLac promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2383
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None